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Six years ago I fell in love with the Alien franchise when I was forced to tackle the Alien Anthology Blu-ray box set in my complete review of it all here. The History of Middlesex County 1635-1885 J. H. Beers & Co., 36 Vesey Street, New York 1884 Pages 229-241 Town of Portland By Hon. William H. BUELL. BibMe Free Bibliography & Citation Maker - MLA, APA, Chicago, Harvard. "The Immortal Life Of Henrietta Lacks" - Journalist Rebecca Skloot (Rose Byrne) accompanies Deborah Lacks (Oprah Winfrey) to Johns Hopkins Hospital to learn more. He. La - Wikipedia. Scanning electron micrograph of an apoptotic He. La cell. Zeiss Merlin HR- SEM. Multiphoton fluorescence image of cultured He. La cells with a fluorescent protein targeted to the Golgi apparatus (orange), microtubules (green) and counterstained for DNA (cyan). Nikon RTS2. 00. 0MP custom laser scanning microscope. ![]() He. La (also Hela or hela) is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line.[1] The line was derived from cervical cancer cells taken on February 8, 1. Henrietta Lacks, a patient who died of her cancer on October 4, 1. The cell line was found to be remarkably durable and prolific which warrants its extensive use in scientific research.[3][4]The cells from Lacks' cancerous cervical tumor were taken without her knowledge or consent.[5]Cell biologist. George Otto Gey found that they could be kept alive,[6][7] and was able to isolate one specific cell, multiply it, and develop a cell line. As was custom for Gey's lab assistant, she labeled the culture 'He. La', the first two letters of the patient's first and last name; this became the name of the cell line. These were the first human cells grown in a lab that were naturally "immortal", meaning that they do not die after a set number of cell divisions (i. These cells could be used for conducting a multitude of medical experiments - - if the cells died, they could simply be discarded and the experiment attempted again on fresh cells from the culture. This represented an enormous boon to medical and biological research.[4]The stable growth of He. La enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine,[8] and by 1. Jonas Salk developed a vaccine for polio using these cells.[4][9] To test Salk's new vaccine, the cells were put into mass production in the first- ever cell production factory.[1. In 1. 95. 3, He. La cells were the first human cells successfully cloned[1. He. La cells quickly grew in the nascent biomedical industry. Since the cells' first mass replications, they have been used by scientists in various types of investigations including disease research, gene mapping, and effects of toxic substances and radiation on humans.[9] Additionally, He. La cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products.[4]Scientists have grown an estimated 2. He. La cells,[4][1. The He. La cell lines are known to overtake other cell cultures in laboratory settings and it is estimated that He. La cells, at one point, contaminated millions of dollars worth of biological research. History[edit]The cells were propagated by George Otto Gey shortly before Lacks died of her cancer in 1. This was the first human cell line to prove successful in vitro, which was a scientific achievement with profound future benefit to medical research. Gey freely donated these cells along with the tools and processes that his lab developed to any scientist requesting them simply for the benefit of science. Neither Lacks nor her family gave permission to harvest the cells but, at that time, permission was neither required nor customarily sought.[1. The cells were later commercialized, although never patented in their original form. There was no requirement at that time (or at present) to inform patients or their relatives about such matters because discarded material or material obtained during surgery, diagnosis, or therapy was the property of the physician or the medical institution. This issue and Lacks' situation were brought up in the Supreme Court of California case of Moore v. Regents of the University of California. The court ruled that a person's discarded tissue and cells are not his or her property and can be commercialized.[1. At first, the He. La cell line was said to be named after a "Helen Lane" or "Helen Larson".[4][1. Starting in the 1. Lacks family was contacted by researchers trying to find out why the He. La cells had contaminated other cell lines in laboratories.[1. These cells are treated as cancer cells, as they are descended from a biopsy taken from a visible lesion on the cervix as part of Lacks' diagnosis of cancer.[1. He. La cells, like other cell lines, are termed "immortal" in that they can divide an unlimited number of times in a laboratory cell culture plate as long as fundamental cell survival conditions are met (i. There are many strains of He. La cells as they continue to mutate in cell cultures, but all He. La cells are descended from the same tumor cells removed from Lacks. The total number of He. La cells that have been propagated in cell culture far exceeds the total number of cells that were in Henrietta Lacks' body.[1. Use in research[edit]He. La cells were used by Jonas Salk to test the first polio vaccine in the 1. They were observed to be easily infected by poliomyelitis, causing infected cells to die.[1. This made He. La cells highly desirable for polio vaccine testing since results could be easily obtained. A large volume of He. La cells were needed for the testing of Salk’s polio vaccine, prompting the National Foundation for Infantile Paralysis (NFIP) to find a facility capable of mass- producing He. La cells.[2. 0] In the spring of 1. Tuskegee University to supply Salk and other labs with He. La cells.[2. 1] Less than a year later, Salk’s vaccine was ready for human trials.[2. He. La cells were also the first human cells to be successfully cloned in 1. Theodore Puck and Philip I Marcus at the University of Colorado, Denver.[2. Since that time, He. La cells have "continually been used for research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits."[9] According to author Rebecca Skloot, by 2. He. La, and that number was increasing steadily at a rate of more than 3. He. La cells have been used in testing how parvo virus infects cells of humans, He. La, dogs, and cats.[2. These cells have also been used to study viruses such as the oropouche virus (OROV). OROV causes the disruption of cells in culture, where cells begin to degenerate shortly after they are infected, causing viral induction of apoptosis.[2. He. La cells have been used to study the expression of the papillomavirus E2 and apoptosis.[2. He. La cells have also been used to study canine distemper virus' ability to induce apoptosis in cancer cell lines,[2. He. La cells have also been used in a number of cancer studies, including those involving sex steroid hormones such as estradiol, estrogen, and estrogen receptors, along with estrogen- like compounds such as quercetin and its cancer reducing properties.[2. There have also been studies on He. La cells, the effects of flavonoids and antioxidants with estradiol on cancer cell proliferation. He. La cells were used to investigate the phytochemical compounds and the fundamental mechanism of the anticancer activity of the ethanolic extract of mango peel (EEMP). EEMP was found to contain various phenolic compounds and to activate death of human cervical malignant He. La cells through apoptosis, which suggests that EEMP may help to prevent cervical cancer as well as other types of cancers.[2. In 2. 01. 1, He. La cells were used in tests of novel heptamethine dyes IR- 8. PDT, co- administration with other drugs, and irradiation.[3. He. La cells have been used in research involving fullerenes to induce apoptosis as a part of photodynamic therapy, as well as in in vitro cancer research using cell lines.[3. Further He. La cells have also been used to define cancer markers in RNA, and have been used to establish an RNAi Based Identification System and Interference of Specific Cancer Cells.[3. Analysis[edit]Telomerase[edit]The He. La cell line was derived for use in cancer research. These cells proliferate abnormally rapidly, even compared to other cancer cells. Like many other cancer cells,[3. He. La cells have an active version of telomerase during cell division,[3. In this way, the cells circumvent the Hayflick limit, which is the limited number of cell divisions that most normal cells can undergo before becoming senescent. Chromosome number[edit]Horizontal gene transfer from human papillomavirus 1. HPV1. 8) to human cervical cells created the He. La genome, which is different from Henrietta Lacks' genome in various ways, including its number of chromosomes. Test if your Windows XP PC can run Windows 7 or the latest Windows 8.1 without issues using these two free tools from Microsoft. Move Current Hard Drive to a New Windows XP System. This article examines moving the hard drive(s) from a system that has Windows XP installed to a new system. Windows XP Clean Install - Step 2 of 34. 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As today’s Nice Price or Crack Pipe ’11 attests however, the first generation was far less of an aesthetic. ![]() Activation Need Speed Hot Pursuit Crackers IndianapolisHealth advocates have focused on the prevalence of advertising for calorie- dense low- nutrient foods as a significant contributor to the obesity epidemic. This research tests the hypothesis that exposure to food advertising during television viewing may also contribute to obesity by triggering automatic snacking of available food. Design. In Experiments 1a and 1b, elementary- school- aged children watched a cartoon that contained either food advertising or advertising for other products and received a snack while watching. In Experiment 2, adults watched a television program that included food advertising that promoted snacking and/or fun product benefits, food advertising that promoted nutrition benefits or no food advertising. The adults then tasted and evaluated a range of healthy to unhealthy snack foods in an apparently separate experiment. Main Outcome Measures. Amount of snack foods consumed during and after advertising exposure. Results. Children consumed 4. Adults consumed more of both healthy and unhealthy snack foods following exposure to snack food advertising compared to the other conditions. In both experiments, food advertising increased consumption of products not in the presented advertisements, and these effects were not related to reported hunger or other conscious influences. Conclusion. These experiments demonstrate the power of food advertising to prime automatic eating behaviors and thus influence far more than brand preference alone. Keywords: Food advertising, Priming, Eating behavior, Children, Obesity. According to the U. Activation Need Speed Hot Pursuit Crackers LoveS. Surgeon General, “Obesity is the fastest growing cause of disease and death in America” (Carmona, 2. And the crisis is not unique to the U. S.; according to the World Health Organization (2. The trend is especially disturbing among young people. Over the past 3. 0 years, the percentage of children and adolescents in the U. S. who are overweight or at risk of becoming overweight has more than tripled to 3. Ogden, et al., 2. This obesity crisis has been fueled by reductions in physical activity, as well as overconsumption of foods high in fat and sugar (Institute of Medicine (IOM), 2. Health authorities believe that the accumulation of unhealthy messages communicated to children through food advertising is a leading cause of unhealthy consumption (Brownell & Horgen, 2. IOM, 2. 00. 6). Every day, children view, on average, 1. Federal Trade Commission, 2. Powell, Szczpka, Chaloupka, & Braunschweig, 2. Moreover, food advertising to children portrays unhealthy eating behaviors with positive outcomes. Snacking at non- meal times occurred in 5. Harrison & Marske, 2. In addition to good taste, the most common product benefits communicated include fun, happiness and being “cool” (Folta, Goldberg, Economos, Bell, & Meltzer, 2. Harrison & Marske, 2. A number of reviews have examined the research on advertising to children and conclude that food advertising leads to greater preferences and purchase of the products advertised (Hastings et al., 2. IOM, 2. 00. 6; Story & French, 2. In addition, as assessed through correlational and quasi- experimental studies, heavier media viewing often predicts more unhealthy diets and higher body weight among children (see IOM, 2. A few studies have also examined effects of food advertising on actual eating behaviors, usually assessed by food choices following exposure to advertising (see Hastings et al., 2. IOM, 2. 00. 6). One study with high ecological validity exposed children at an overnight camp to a daily cartoon with candy or fruit advertising, PSAs, or no ads (Gorn & Goldberg, 1. Over a 2- week period, children who saw the candy ads selected fruit and orange juice as a snack less often than the other children. The literature reviews also highlight, however, the need for further research - - specifically, more studies that establish a direct causal link between food advertising and unhealthy diets. To begin to address this need, Halford and colleagues recently demonstrated that groups of children eat more immediately after viewing a series of 8–1. Halford, Boyland, Hughes, Oliveira, & Dovey, 2. Halford et al., 2. Halford, Gillespie, Brown, Pontin, & Dovey, 2. Additionally, these effects occurred at the category level, (i. However, the authors did not obtain support for their proposed mechanism: specifically, that overweight children have greater recognition memory for food advertisements, which in turn leads to greater consumption. The literature reviews also emphasize the need to extend food advertising research beyond children; to- date, very little is known about such effects on adolescents and adults. Finally, most research has examined advertising for calorie- dense, low- nutrient foods. As a result, we know very little about how advertising for more nutritious food affects eating behaviors. The present research addresses these gaps in our knowledge and utilizes a new approach to study food advertising effects using contemporary social- cognitive theories. Advertising as a “real- world” prime. Social- cognitive theories suggest a subtle and potentially far- reaching effect of food advertising on eating behaviors that may occur outside of participants’ intention or awareness (i. Bargh & Morsella, 2. Priming methods provide a means to test for these automatic causal effects. In priming studies, relevant mental representations are activated in a subtle, unobtrusive manner in one phase of an experiment, and then, the unconscious, unintended effects of this activation are assessed in a subsequent phase (see Bargh & Chartrand, 2. Priming research has already demonstrated that a variety of complex social and physical behaviors – such as aggression, loyalty, rudeness, and walking speed – can be activated by relevant external stimuli (i. Dijksterhuis, Chartrand, & Aarts, 2. The mechanism through which behavior priming operates appears to be an overlap or strong association between representations activated by the perception of a given type of behavior, and those used to enact that type of behavior oneself (Dijksterhuis & Bargh, 2. Bargh, 2. 00. 5; Chartrand & Bargh, 1. Tomasello, Call, Behne, & Moll, 2. An important real- life source of priming influences is the media, including television programs and advertisements. Exposure to aggressive or alcohol- consuming models in media can prime aggressive behaviors and alcohol consumption in the viewer (see Anderson & Bushman, 2. Roehrich & Goldman, 1. Studies that have focused specifically on advertising effects have shown that ads can prime positive expectancies of the effects of alcohol consumption (Dunn & Yniguez, 1. Pechman & Knight, 2. External cues and consumption behaviors. Research among adults confirms that external cues have a significant influence on food consumption behaviors. Exposure to the sensory properties of palatable food increased subjective desire and consumption, even though participants were already fully sated (Cornell, Rodin & Weingarten, 1. Subsequent studies confirmed and extended this finding, showing that exposure to sensory- related food cues increases consumption (Federoff, Polivy & Herman, 1. Jansen & van den Hout, 1. Rogers & Hill, 1. Moreover, food advertising typically focuses on the immediate sensory gratifications of consumption (i. Loewenstein, 1. 99. Metcalfe & Mischel, 1. In light of these findings, Lowe and Butryn (2. Consumption behaviors can also be activated through automatic processes. External cues, not related to the sensory qualities of food, (e. Wansink, 2. 00. 6). The behavior of other people is another important external behavioral cue, and people automatically mimic others’ eating behaviors, including food choice and amount of food consumed, without realizing they are doing so (Johnston, 2. Tanner, Ferraro, Chartrand, Bettman & van Baaren, in press). The unconscious nature of these influences is further established by studies in which primes of thirst- related words or smiling faces, presented subliminally, outside of the participant’s conscious awareness, increased beverage consumption among thirsty individuals (Strahan, Spencer & Zanna, 2. Winkielman, Berridge, & Wilbarger, 2. Food advertising. 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If you’re out and about, turn the Wi- Fi off and just use cellular data to conserve some battery life. All of this can be done from the Control Centre, which is accessed by swiping up from the bottom of your screen. It’s also advisable to turn off Background App Refresh by going to Settings > General > Background App Refresh. This will give you the option to stop specific apps updating in the background, saving you a load of battery life in the process. Related: i. Phone 7 review. And if you want to see which of those apps is draining your phone’s cell the most, head over to Settings > Battery to see a list of the biggest offenders. Finally, you can try turning off fitness tracking by going to Settings > Privacy > Motion & Fitness. If you don’t want to track your fitness, this can be a great way of conserving that precious battery life – especially if you’ve got an Apple Watch, which does all the tracking for you anyway. Bluetooth and Wi- Fi aren’t working in i. OS 1. 0You can bet there’ll be a few problems with wireless technology and i. OS 1. 0, at least at first. Whether it’s Wi- Fi or Bluetooth, Apple’s OS usually has the odd problem when it first launches. Related: i. Phone 8. If you’re having trouble with either, just go to Settings > General > Reset > Reset Network Settings. This should reset all your wireless connections to default settings without deleting your data. My device is overheating now I’ve got i. OS 1. 0When you first download and install i. OS 1. 0, it’ll continue updating apps in the background. This means your phone or tablet may heat up more than usual when you charge it. However, this should only be a temporary problem, and once all the apps have been updated, you should notice the device return to normal temperatures during charging. If you’re still having problems, you might consider trying a hard reset or turning off auto- sending of diagnostic and usage data by going to Settings > Privacy > Diagnostics & Usage. If none of that works, it might be time to visit the Apple Store or contact Apple customer support. OS 1. 0 is running slower than i. OS 9. You may find that i. OS 1. 0 runs slower than i. OS 9, in which case the problem could be down to a variety of things. Firstly, as with the overheating- during- charging issue, you’ll find that the operating system speeds up the longer you leave it. That’s because i. OS 1. 0 re- indexes everything and optimises apps to work with the new software in the background after you’ve installed it. Once it’s finished – the process should take a few hours – things should speed up. Related: How to uninstall i. OS 1. 0But if, after leaving the phone overnight, you’re still unhappy with the OS’s performance, you can try a few other things. Firstly, try turning on Reduce Motion by going to Settings > General > Accessibility > Reduce Motion. This essentially turns off all the special motion effects, making the OS run smoother. Similarly, turning on Reduce Transparency should make things run a bit slicker. Access this in Settings > General > Accessibility > Increase Contrast. From there, turn the Reduce Transparency setting on. You can also try turning off Background App Refresh, which is detailed in the Battery section of this article. Freeing up storage space is a good way to ensure the OS runs smoothly, too. Try deleting old files and photos, and ensuring you have at least 1. GB of free space on your phone’s memory. If you’ve got a 1. GB i. Phone, we feel for you. If all else fails, give the old ‘turn it off and on’ trick a try, before resorting to a system restore or factory reset. Otherwise it’s a case of contacting Apple. OS 1. 0 is taking forever to download. Finally, you might find i. OS 1. 0 is taking a while to download. If you’re struggling to get the new OS downloaded, try the usual fixes first – the most obvious of which is to switch from cellular to Wi- Fi if you’re trying to download the new software over a data connection. Alternatively, it might be better to connect your i. Phone or i. Pad to i. Tunes and download the update that way. Related: i. Phone 7 problems – and how to quickly fix them. WATCH: i. OS 1. 0 hands- on. Got any other tips or suggestions? Let us know in the comments. LG G3 Support | Verizon Wireless. Apple® i. Phone® 8 Plus. Apple® i. Phone® 8. Apple® Watch Series 3. Apple® i. Phone® 6 Plus. Apple® i. Phone® 6. Apple® i. Phone® 7. Apple® i. Phone® 6s. Apple® i. Phone® 7 Plus. Verizon Ellipsis™ Jetpack®. Verizon Jetpack® 4. G LTE Mobile Hotspot Mi. Fi® 5. 51. 0L. Moto Z Play Droid Edition. Samsung Galaxy J3 Eclipse. Moto Z Force Droid Edition. Verizon Jetpack® Mi. Fi® 6. 62. 0L. Samsung 3. G Network Extender. Samsung Galaxy J1™ Prepaid. Jetpack® 4. G LTE Mobile Hotspot Mi. Fi® 4. 51. 0L. Droid Turbo in Ballistic Nylon. Verizon Wireless Home Phone. Samsung 4. G LTE Network Extender. Samsung Galaxy Core Prime™. Verizon Jetpack® 4. G LTE Mobile Hotspot - AC7. L. Ellipsis™ Jetpack® MHS8. L. Verizon Mi. Fi® 4. G LTE Global USB Modem U6. L. Pantech 4. G LTE Global USB Modem UML2. Verizon 4. G LTE Broadband Router. Verizon LTE Internet and Home Phone. Verizon Jetpack® 4. G LTE Mobile Hotspot 8. L. LG Optimus Zone™ 3 Prepaid. Dura. Force PRO with Sapphire Shield. Verizon Jetpack® 4. G LTE Mobile Hotspot Mi. Fi® 4. 62. 0L. Verizon 4. G LTE USB Modem 5. L. Verizon Verizon Smart. Hub with Voice. Verizon Ellipsis® Jetpack® MHS9. L. Verizon 4. G LTE USB Modem UML2. Ellipsis™ Jetpack® MHS8. L. Black. Berry® Bold™ 9. Casio G'z. One Commando® 4. G LTE. Verizon Global USB Modem USB7. L. Galaxy J3 Mission Prepaid. Verizon Jetpack® 4. G LTE Mobile Hotspot Mi. Fi® 4. 62. 0LE. Verizon Wireless Home Phone T2. Verizon Jetpack® 4. G LTE Mobile Hotspot MHS2. L. Verizon Wireless Air. Card® 5. 95. Samsung Jetpack® 4. G LTE Mobile Hotspot SCH- LC1. Motorola moto e. 4 plus™ Prepaid. Verizon Mi. Fi™ 2. Intelligent Mobile Hotspot - OTA. Apple® i. Pad® mini with Retina display. DROID TURBO 2 - Designed by You. Apple® i. Pad Pro® (1. Black. Berry® Z1. Black. Berry® Q1. Apple® i. Pad Pro® (9. Verizon Wireless USB7. Modem. DROID INCREDIBLE 4. G LTE by HTC. Apple® i. Pad Pro® (1. 2. 9- inch). Samsung Galaxy Tab® 2 (7. Verizon Mi. Fi™2. Intelligent Mobile Hotspot. Black. Berry® Z3. Samsung Galaxy Tab® 2 (1. Apple® i. Pad® with Wi- Fi + 4. G. DROID RAZR MAXX HD by MOTOROLA. LG 4. G LTE USB Modem VL6. Samsung Galaxy Tab® (1. Black. Berry® Bold 9. LG Optimus Zone™ 2 Prepaid. Samsung Galaxy Tab® 4 (1. Samsung Galaxy S® III mini. HTC One® (M8) for Windows®. Samsung Galaxy Tab® 4 (8. Black. Berry® Tour™ 9. Verizon Wireless USB7. Modem. Delphi Connect with 4. G LTE Mobile Hotspot. Verizon Fivespot™ Global Ready™ 3. G Mobile Hotspot. Black. Berry® Bold™ 9. DROID RAZR MAXX by MOTOROLA. Apple® i. Pad® with Retina display. Verizon Wireless UMW1. Global USB Modem. DROID RAZR HD by MOTOROLA. Verizon Wireless Air. Card® 5. 95. U USB Modem. Verizon Wireless UM1. USB Modem. Black. Berry® Storm. 2™ 9. DROID INCREDIBLE 2 by HTC. Samsung Galaxy™ Stratosphere™ II. Watch Urbane™ 2nd Edition LTE. Samsung Galaxy Note® 1. Samsung Galaxy Note® 1. Edition. Samsung Galaxy™ Stratosphere™. Apple® i. Pad® with Wi- Fi and Mi. Fi® 2. 20. 0 Hotspot. Samsung Galaxy Tab® (7. Verizon Wireless USB7. Modem. Black. Berry® 8. World Edition smartphone. Black. Berry® Curve™ 9. Verizon Wireless G'z. One Type- S. Black. Berry® Curve™ 8. 53. Verizon Wireless UM1. USB Modem. DROID 2 GLOBAL by MOTOROLA. Verizon Wireless PC5. PC Card. Black. Berry® Curve™ 9. Moto X™ - Designed by You. Moto X™ - Designed by You (2nd Generation). Black. Berry Classic Non- Camera. DROID XYBOARD 1. 0. MOTOROLA. Verizon Wireless Escapade®. Verizon Wireless G'z. One Type- S Push to Talk without Camera. DROID XYBOARD 8. 2 by MOTOROLA. Verizon Wireless V7. Express. Card. Sierra Wireless Air. Card® 5. 55. D. Sierra Wireless Air. Card® 5. 80. Luge™ by Motorola Prepaid. Nokia 2. 70. 5 Shade™ Non- Camera. Motorola RAZR V3m in Gray. Motorola MOTO™ Z6c World Edition. Verizon AD3. 70. 0 Global USB Modem. G'z. One Boulder™ Black and Silver. Casio G'z. One Ravine® 2 Non- Camera. Verizon Wireless USB1. Global USB Modem. Motorola MOTO™ Z6cx World Edition without Camera. Black. Berry® Curve™ 9. Verizon Wireless PC7. Card and Express. Card®. HP® Mini 1. NR Netbook. Compaq® Mini CQ1. Netbook. HP® Mini 3. NR Netbook. Verizon Converged VOIP Device. HTC Ozone™ with TALKS™ for Verizon Wireless. Verizon Wireless U6. PC Card. HP® Mini 2. NR Netbook. Verizon Wireless Blitz® Prepaid. Verizon XU8. 70 Global Express. Card®. Motorola MOTORAZR maxx Ve. HP® Pavilion dm. 1- 3. Notebook. HP® Pavilion dm. Entertainment Notebook. Verizon Wireless Adamant™. 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